Inactive - CU Cancer Center Molecular Pathology/Cytogenetics Shared Resource

Mission Statement:

The CU Cancer Center Molecular Pathology/Cytogenetics Laboratory provides access and expertise to classical and molecular technologies. Services range from the karyotypic characterization of specimens by G-banding staining to the development of fluorescence in situ hybridization (FISH) reagents.

 

The lab also works on projects aiming to identify and validate diagnostic and prognostic molecular markers, as well as predictive markers for sensitivity and resistance to targeted therapies in solid tumors. 

 

Consulting services for experimental design and data interpretation and specialized training for non-assisted lab use are other services offered.

How to use this service lab:

Usually arrangements for regular users are made by email or by phone, directly with the unit director or designated technical personnel. For new users, for projects with different goals for regular users, or consultation on strategies to address scientific questions face-to-face meetings with the unit director are highly recommended.

 

Turnaround time (TAT) is highly dependent on the specimen or project. Short projects are accepted and performed on a 1st-come, 1st-served basis. Large projects must be discussed and scheduled with the lab director in order to optimize the TAT.

 

Studies involving human tissues require appropriate IRB approval or exemption.

 

Specimens can be directly delivered by a lab member from the user laboratory or shipped via common methods.

 

Use of instrumentation is available by appointment only and SR technologists have priority during their work period.

 

Please contact Dr. Varella-Garcia to schedule your project and discuss expected lab TATs and investigators’ needs.

Services Available:

The MPSR/Cytogenetics uses a user fee system whereby users are charged based on the services used. 

 

Pricing is established through an annual cost study that is evaluated by the UCCC Administrative Office and the UCD Finance Department. The cost study defines the total operating costs and establishes the subsidies on services that UCCC members will receive as a result of the CCSG funding.

 

Services and their average prices are posted on the 'Request Services' tab of this site. Since labor is an essential component of cost, simple analyses may cost less and complex analyses may cost more than average. Better estimates of final cost for individual projects can be obtained with the lab director.

Services:

• Cell culture and harvesting
• Tailored Slide making
• Ploidy Evaluation
• Classical Karyotyping
• Multicolor Karyotyping
• DNA Cloning
• DNA Amplification
• DNA Labeling
• FISH assays – probe A (homebrew)
• FISH assays – probe B (commercial)
• Consulting

Leadership:

Marileila Varella-Garcia, PhD

Director and Professor

marileila.garcia@ucdenver.edu

303-724-3147

Location:

Anschutz Medical Campus
12801 E. 17th Avenue

RC1-South, Rooms 8401A, B, C and 8202
Aurora, CO  80045

Hours:  8:00am – 5:00pm M-F

Phone: 303-724-3148

Fax:     303-724-3889

Mailing Address:

Cancer Center Molecular Pathology/Cytogenetics Service

University of Colorado Anschutz Medical Campus

12801 E 17th Avenue

RC1-South, Rooms 8401A, B,C and 8202

Campus Box 8117

Aurora, CO  80045

Links and Resources:

• Molecular Pathology and Cytogenetics Site

Contacts

Name	Role	Phone	Email	Location
Marileila Varella-Garcia, PhD	Director and Professor	303-724-3147	marileila.garcia@ucdenver.edu	RC1-South, L18-8118
Jamie Sheren, PhD, CG(ASCP)	Lead Scientist for Technical Operations	303-724-3148	jamie.sheren@ucdenver.edu	RC1-South, L18-8401A
Aline Murakami-Walter, MS	Technician	303-724-3148	aline.murakamiwalter@ucdenver.edu	RC1-South, L18-8401A
Ying Wang, PhD	Technician	303-724-3148	ying.2.wang@ucdenver.edu	RC1-South, L18-8401A