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Cell Technologies Shared Resource
The University of Colorado Cancer Center Cell Technologies Tissue Cultrue Shared Resource (CTSR) provides services to the University of Colorado Anschutz Medical Campus and the surrounding academic and industrial community.CEll Technologies Shared Resource (CTSR) supports basic and translational research with validated cell lines, preparation of hybridomas, production of monoclonal antibodies, expressing recombinant protein in baculovirus and mammalian systems, large scale Mab production and purification, and live cell imaging on Sartorius Incucyte S3 system.
PPSR provides centralized services to produce cancer-related proteins for CU Cancer Center members to study their mechanism of action in identified drugs and cancer-related biomarkers.
Steven Anderson, PhD Director
Anh Le CTSR Manager
| Hours | Location |
|
Monday - Friday 8:00am-4:00pm |
12801 E. 17th Ave. Anschutz Medical Campus |
Please click here to access our website.
University of Colorado Cancer Center
ATTN: Barbara Johnson
13001 E. 17th Place, Room C6004P
Mailstop F434, Building 500
Aurora, CO 80045
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Anh Le, BA |
Manager
|
303-724-3770
|
anh.le@cuanschutz.edu
|
RC-1 S 5404b
|
| Steve Anderson, PhD |
Director
|
303-724-3742
|
steve.anderson@cuanschutz.edu
|
RC-1 S 5110
|
| Prices* |
| ► Baculovirus (7) | |||
| Name | Description | Price | |
|---|---|---|---|
| Bacmid Transfection | Inquire | ||
| Titer 1 Virus | Inquire | ||
| Plaque Purify Existing Viral Stock | Inquire | ||
| 500 ml viral stock | Inquire | ||
| 500 ml protein production | Inquire | ||
| Each Additional L | Inquire | ||
| 2L Wave start up |
|
Inquire | |
| ► Mammalian PEI Transient Transfection for Mab or Protein Production (3) | |||
| Name | Description | Price | |
| Mammalian PEI Transient Transfection for Protein or Mab 1 Liter | Inquire | ||
| Mammalian Transient Transfection for Protein/Mab-Addition cost /L for Large Scale Growth | Inquire | ||
| Additional Cost using Commercial Transfection Reagent/1L | Inquire | ||
| ► Monoclonal Antibody Production (2) | |||
| Name | Description | Price | |
| 1L Hybridoma Supt/Cell Line Growth | Inquire | ||
| Mab Supt Purification (1-5L) | Inquire | ||
| ► Cell Culture (7) | |||
| Name | Description | Price | |
| Cell line derivation | Inquire | ||
| CUTO cell line request | Inquire | ||
| Cell lines | Inquire | ||
| Mycoplasma Test-Lonza Myco Alert test | Inquire | ||
| STR Analysis & Authentication of HUMAN Cell Lines |
Currently the Cell Technologies Shared Resource can only authenticate human cell lines |
Inquire | |
| Freeze 1 Cell Line for LN2 Storage | Inquire | ||
| LN2 Storage Rental for 1 section in LN2 Tank (per year) | Inquire | ||
| ► Fusions (4) | |||
| Name | Description | Price | |
| Fusion including ELISA screenings and mouse injections | Inquire | ||
| Cloning and Freeze 1 positive hybridoma | Inquire | ||
| Isotyping (up to 12 samples) | Inquire | ||
| Freeze additional 1 polyclonal-2 ampules | Inquire | ||
| ► Protein Purification (2) | |||
| Name | Description | Price | |
| Gel Filtration Protein Purification |
Gel filtration (GF) chromatography separates proteins solely on the basis of molecular size. Separation is achieved using a porous matrix to which the molecules, for steric reasons, have different degrees of access--i.e., smaller molecules have greater access and larger molecules are excluded from the matrix. |
Inquire | |
| Mono Q Column Protein Purification |
Mono Q are strong anion exchange chromatography columns for protein high resolution polishing of proteins |
Inquire | |
