CU Cancer Center Flow Cytometry Shared Resource

*** Welcome to the Cancer Center Flow Cytometry Shared Resource ***

 Please remember to acknowledge the Cancer Center Support Grant (P30CA046934), the Skin Diseases Research Cores Grant (P30AR057212), and our Flow Cytometry Shared Resource in any publication resulting from this service.

• For easy appointment scheduling choose the Reservations Tab! Under "Select Resources" on the right hand side of the screen, check any instruments plus staff members you need. You will be able to easily see where there are both staff and equipment openings. To make an appointment on the instrument, click the name of the instrument at the top of the column. We love to work with Researchers, Please don't hesitate to contact us if you are having difficulties of any kind! cc.flowcyto@ucdenver.edu is our lab email that reaches all of us.

• All Appointments may be done in Person.  We encourage everyone to consider training to operate the instruments if possible. Teamviewer is still an option for those who would like to operate the analyzers remotely after setting up their experiment.

• For Assisted runs, Samples may be dropped off.
• Bring samples for the Sorters to RC1-South, 4th floor, Room 4215
• Bring samples for the Analyzers in the CC FCSR facility in RC1-South 4th floor
• If you are early, drop your samples in the FCSR Refrigerator next to the dishroom (TOP SHELF), and email cc.flowcyto@ucdenver.edu that you have dropped off your samples.
• If you are on time/late, bring your samples to the instrument room
• CyTOF XT 4208
• ZE-5 (YETI) 4208
• Penteon 4204
• Gallios 4204
• Bring samples for the Analyzers in the CC-IMM facility in RC1- North 8th floor
• Arrange with the staff member helping you if you are going to be early or late by emailing cc.flowcyto@ucdenver.edu as soon as possible. 
• CC Aurora 8207
• IMM Aurora 8207
• IMM Northern Lights 8207
• IMM Fortessa 8207

• Sample Safety for all who use our facility is very important. Please do not bring BSL3 samples to our facility. We can handle up to BSL2+ with proper notification. 

• BSL-2 includes but is not limited to:
• Human Blood, Plasma, PBMCs, Bone Marrow, and other Non-Respiratory/tissues/cells/fluids with the exception of GI tissues
• BSL-2+ includes but is not limited to:
• Human Upper and Lower Respiratory samples (nasal, lung, sputum,) and GI tissues or any feces

• Please fill out your iLab appointment requests accurately so that we may best prepare for your appointments and samples.

*** Thank you!!!   :) ***

 Overview of Services

The University of Colorado Cancer Center Flow Cytometry Shared Resource provides flow cytometry services to the University of Colorado Anschutz Medical Campus and the surrounding academic and industrial community. The FCSR also serves as the Flow Cytometry Core for the Gates Stem Cell Center.

Using flow cytometric analysis, high-speed cell sorting, and multiplexed fluorescent microsphere assays, the Cancer Center Flow Cytometry Shared Resource (FCSR) can perform a wide range of assays, including cell phenotyping, cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, and fluorescent protein analysis.

Equipment

Analysis Flow Cytometers

• Standard Biotools CyTOF XT Mass Cytometer (see Mass Cytometry)
• Agilent Novocyte Penteon cytometer
• Cytek Aurora spectral cytometer
• Cytek Northern Lights spectral cytometer
• Bio-Rad ZE-5 (YETI)
• BD Biosciences Fortessa
• Beckman Coulter Gallios with 561 laser

Cell Sorters

• Sony MA 900 (70, 100, or 130 um chip)
• Miltenyi Tyto cartridge based Cell sorter (Regular and High Speed Cartridges are available)
• Beckman Coulter Astrios (70 tip)
• Beckman Coulter XDP Flex (100 or 70 tip)
• Beckman Coulter XDP 100 (100 tip)

Mass Cytometry

• Standard Biotools CyTOF XT Mass Cytometer

Additional Services

• ViCell Blu cell counter
• HIMSR-FCSR joint Mass Cytometry Antibody Bank 
• Analysis Computers
• Analysis Software Site Licenses
• FCS Express
• FlowJo
• Cell Engine
• Kaluza
• Astrolabe
• Experimental and Panel Design Assistance (Ask us before ordering your Antibodies!)
• Cell Cycle Analysis
• Figures for Publication
• Data Analysis including High Parameter analysis/assistance
• Flow Cytometry reagents (compensation beads, live/dead dyes, fixative)
• FlowSet Pros to standardize experiments

Billing Policies

• All analysis appointments are charged a minimum of 1/2 hour and in increments of 15 minutes thereafter. 
• All sorting is charged a minimum of 1 hour and in in crements of 15 minutes thereafter
• You will be charged for the time booked or the time used, whichever is greater. Late appointments are charged from the beginning of the scheduled appointment time.

Late Arrivals, No-Shows, Failure to use the Kiosk, & Cancellations

• Please arrive promptly (or early! if the instrument is available) for your appointment. Log in and out of the Kiosk to accurately record your usage.
• Early arrivals will be charged for time booked or time used, whichever is greater (we will never penalize early runs!)
• Late arrivals will be charged from the beginning of their scheduled appointment if you email cc.flowcyto@ucdenver.edu at least 1 hour before your self-run appointment time, we can assist you in moving your appointment to a later time. This is usually not possible with assisted appointments.)
• Failure to login/out with the iLabs Kiosk (for self-run) or No-Shows for appointments will be charged the time scheduled + an assisted use add on for the time it takes to track down the time used/not used. 
• To avoid this add on charge, email cc.flowcyto@ucdenver.edu the time used or that you were unable to make your appointment. If it is an hour or more before your appointment (selfrun only) we can move your appointment to a future time or date.
• If you need to cancel your appointment - cancel as soon as you know you need to cancel, email cc.flowcyto@ucdenver.edu when you cancel your appointment.
• Assisted Appointments
• Sorter appointments 
• 3 or more working days
• No Charge
• Another Researcher uses the time you were scheduled for
• No Charge
• Less than 3 working days
• Time scheduled will be charged
•  Analyzer Appointments
• 1 or more working days
• No Charge
• Another Researcher uses the time you were scheduled for
• No Charge
• Less than 1 working day
• Time scheduled will be charged
• Self-run Appointments are similar to the Assisted Appointments, but we have reduced the cancellation time. If no email is sent, the times remain the same as the Assisted Appointments.
• Sorter appointments
• 1 or more working days + email to cc.flowcyto@ucdenver.edu
• No Charge
• Analyzer appointments
• 8 or more hours + email to cc.flowcyto@ucdenver.edu 
• No Charge

Leadership

Eric Clambey, PhD   Director

Christine Childs, MT(ASCP) Manager

Location and hours of operation

Please note that the instruments are housed in multiple rooms: ( RC1- South 4204, 4208, 4215, 4402A, RC1-North 8207)

Links and Resources

Please click here for a FlowJo or FCS Express subscription. 

Please use this link to access our core website.

Contacts

Available Equipment and Resources

Map